Here you are a simple homework to practice with Primer3 and our genome finishing goal.
Using the information stored in mate-paired reads, we see that the 5′-end of “contig05189” is linked to 3′-end of “contig01316”. Please note the 3′ and 5′ ends are indicated in gray boxes for each connection.
You can see that the path to the 3′ is a little bit more complex.
