Genome sequencing and scaffolding and gaps…
Introduction to bioinformatics analysis. Simple HTML file.
Perl crash course intro: variables and for/foreach cycles.
Some more examples on scalar and arrays. Hashes.
Operators and conditionals, “if” statement.
On March we’ll meet at the ex-Taliercio lab these afternoons (14.30 – 18.30 unless otherwise stated):
Shell commands (filesystem, a little bit advanced and possibly using Tab),
Manual primer design with Primer3 on line (homework),
First Perl scripts: variables, for cycles.
Starting in Aula B Pr (Vallisneri).
Command line tools: BLAST and Primer3,
Perl scripts: opening files and executing commands.
Starting our pickprimer.pl program
Subroutines and running programs via Perl.
pickprimer.pl starts working!
Blasting primers to select the most specific primers pair.
2012-04-17 and 18
Wet lab practical: PCR amplification of selected gaps. Gel electrophoresis, and preparation of samples for Sanger sequencing.