## First practical

Welcome to the first practical. During this practical you’ll learn how to master the command line and Linux as well.
Please, keep in mind that all the tools we are presenting day by day are necessary to fulfil the final assignment, so try to think about all the steps rather than rushing to the end.

Feel free to ask us, in case of troubles…

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## Calculate your oligonucleotide resuspension volume

As promised during today’s lecture, here you have your homework:

please calculate the amount  of water to be added to each of the primers in the list, in order to produce a working solution of 10 µM. You can produce a concentrated stock solution and then dilute the stock solution to a working solution of 10 µM, if it is needed.

Oligonucleotide datasheet containing all the information as provided by the supplier

Did you managed to find the right volume for each stock solution?

The Hamilton pipetter needs you to provide a coordinate and a volume separated by a tab for each of the primer. The information concerning each of the primers must be separated by a new line character, as shown in the example below

example of coordinate's file that you should pass to the Robot, to guide it (him?) doing the pipetting for you

Now try to translate the work that you performed to get this result into a list of actions (the algoritm).

For the most willing students, this is the moment for translating your algoritm into a Perl program and testing it at volume calculation for this short primer’s list.

Very soon you will find the solution on this blog. Stay tuned

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## Lab Groups

In attachment to this post you will find the list of students divided in 7 groups. There are 5 groups of 4 students and 2 groups of 3 students. You can change group as long as you find a friend who agrees to perform the exchange and as long as the exchange fits these three simple roules:

• groups number 1, 2, 3, 4 and 5 must be composed of 4 people
• groups number 6 and 7 must be composed of 3 people
• Annagiulia, Tea and Ilaria must not be moved from the assigned group
remember to bring you lab coat and a calculator with you
see you soon in the labs

groups list

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